| Team Name | Enroll
-ment | Class | Head School | Co-oping Schools
(if Applicable) | | Ankeny | 2724 | 3A | Ankeny | Ankeny Centennial
Ankeny Christian Academy
| | Bellevue | 163 | 1A | Bellevue | | | Benton Community | 381 | 1A | Benton Community | | | Bettendorf | 1252 | 3A | Bettendorf | Morning Star Academy, Bettendorf
Rivermont Collegiate, Bettendorf
| | Bondurant-Farrar | 470 | 2A | Bondurant-Farrar | | | Burlington | 746 | 2A | Burlington | | | Camanche | 231 | 1A | Camanche | | | Cedar Falls | 1427 | 3A | Cedar Falls | Dike-New Hartford
| | Cedar Rapids Jefferson | 1249 | 3A | Cedar Rapids, Jefferson | Cedar Valley Christian
| | Cedar Rapids, Kennedy | 1350 | 3A | Cedar Rapids, Kennedy | Cedar Valley Christian
| | Cedar Rapids, Washington | 1055 | 2A | Cedar Rapids, Washington | Cedar Valley Christian
| | Centerville | 299 | 1A | Centerville | | | Central DeWitt | 385 | 1A | Central DeWitt | | | Charles City | 387 | 1A | Charles City | | | Cherokee, Washington | 400 | 1A | Cherokee, Washington | Alta-Aurelia
| | Clarinda | 251 | 1A | Clarinda | South Page, College Springs
| | Clarke of Osceola | 300 | 1A | Clarke, Osceola | | | Clinton | 730 | 2A | Clinton | | | Columbus Catholic, Waterloo | 186 | 1A | Columbus Catholic, Waterloo | | | Council Bluffs, Abraham Lincoln | 1021 | 2A | Council Bluffs, Abraham Lincoln | | | Council Bluffs, Thomas Jefferson | 975 | 2A | Council Bluffs, Thomas Jefferson | | | Creston O-M | 416 | 1A | Creston | Orient-Macksburg
| | Davenport Central | 1519 | 3A | Davenport, Central | Assumption, Davenport
| | Davenport North | 1295 | 3A | Davenport, North | Assumption, Davenport
| | Davenport West | 1506 | 3A | Davenport, West | Assumption, Davenport
| | Davis County, Bloomfield | 283 | 1A | Davis County, Bloomfield | | | Decorah | 472 | 2A | Decorah | | | Denison-Schleswig | 584 | 2A | Denison-Schleswig | | | Denver | 294 | 1A | Denver | Dunkerton
| | Des Moines Abraham Lincoln | 3102 | 3A | Des Moines, Abraham Lincoln | Carlisle
Indianola
| | Des Moines East | 1560 | 3A | Des Moines, East | | | Des Moines, Hoover | 783 | 2A | Des Moines, Hoover | | | Des Moines, North | 1031 | 2A | Des Moines, North | | | Des Moines, Roosevelt | 1566 | 3A | Des Moines, Roosevelt | | | Dowling Catholic, West Des Moines | 1071 | 3A | Dowling Catholic, West Des Moines |
| | Dubuque, Hempstead | 1337 | 3A | Dubuque, Hempstead | | | Dubuque, Senior | 1149 | 3A | Dubuque, Senior | | | Durant | 354 | 1A | Durant | Wilton
| | Emmetsburg | 209 | 1A | Emmetsburg | | | Epworth, Western Dubuque | 674 | 2A | Epworth, Western Dubuque | | | Fairfield | 382 | 1A | Fairfield | | | Forest City | 256 | 1A | Forest City | | | Fort Dodge | 844 | 2A | Fort Dodge | | | Grundy Center | 152 | 1A | Grundy Center | | | Harlan | 383 | 1A | Harlan Community | | | Independence | 339 | 1A | Independence | | | Iowa City High | 2813 | 3A | Iowa City, City High | Solon
Regina, Iowa City
West Branch
Iowa City, Liberty
Lone Tree
| | Iowa City West | 2926 | 3A | Iowa City, West | Regina, Iowa City
Clear Creek-Amana
Iowa City, Liberty
Mid-Prairie, Wellman
| | Isaac Newton Christian Academy | 48 | 1A | Isaac Newton Christian Academy | Cedar Valley Christian
| | Johnston | 1763 | 3A | Johnston | | | Keokuk High School | 697 | 2A | Keokuk | Holy Trinity Catholic, Fort Madison
Central Lee, Donnellson
| | Knoxville | 447 | 2A | Knoxville | Melcher-Dallas
| | Lamoni | 248 | 1A | Lamoni | Central Decatur, Leon
| | LeMars | 501 | 2A | LeMars | | | Lenox | 109 | 1A | Lenox | | | Lewis Central High School | 877 | 2A | Lewis Central | Treynor
| | Linn-Mar, Marion | 1702 | 3A | Linn-Mar, Marion | | | Louisa-Muscatine | 391 | 1A | Louisa-Muscatine | Wilton
| | Maquoketa | 357 | 1A | Maquoketa | | | Marion | 667 | 2A | Marion | Alburnett
| | Marshalltown | 1316 | 3A | Marshalltown | East Marshall, LeGrand
| | Mason City | 1233 | 3A | Mason City | Clear Lake
Rockford
| | MMCRU/RSM | 420 | 1A | MMCRU | St. Mary's, Remsen
Cherokee, Washington
| | Monticello | 301 | 1A | Monticello | | | Mount Ayr | 148 | 1A | Mount Ayr | | | Mount Pleasant | 721 | 2A | Mount Pleasant | New London
Danville
| | Muscatine High School | 1393 | 3A | Muscatine | West Liberty
| | Newton | 638 | 2A | Newton | | | Nodaway Valley | 291 | 1A | Nodaway Valley | Earlham
| | North Fayette Valley | 247 | 1A | North Fayette Valley | | | North Iowa | 78 | 1A | North Iowa, Buffalo Center | | | North Scott, Eldridge | 727 | 2A | North Scott, Eldridge | | | Norwalk | 704 | 2A | Norwalk | | | OA-BCIG | 391 | 1A | OABCIG | Ridge View
| | Oelwein | 338 | 1A | Oelwein | West Central, Maynard
| | Ottumwa | 1145 | 3A | Ottumwa | Cardinal, Eldon
| | Pella | 535 | 2A | Pella | | | Pella Christian | 183 | 1A | Pella Christian | | | Pleasant Valley | 1117 | 3A | Pleasant Valley | | | Prairie, Cedar Rapids | 1627 | 3A | Prairie, Cedar Rapids | Mount Vernon
| | Red Oak | 241 | 1A | Red Oak | | | Sergeant Bluff-Luton | 377 | 1A | Sergeant Bluff-Luton | | | Shenandoah | 269 | 1A | Shenandoah | Essex
| | Sigourney-Keota | 198 | 1A | Sigourney | Keota
| | Sioux Central | 711 | 2A | Sioux Central, Sioux Rapids | Spencer
| | Sioux City, East | 1119 | 3A | Sioux City, East | | | Sioux City, North | 1248 | 3A | Sioux City, North | | | Sioux City, West | 1018 | 2A | Sioux City, West | | | Southeast Polk | 1847 | 3A | Southeast Polk | PCM, Monroe
| | Southeast Warren | 124 | 1A | Southeast Warren, Liberty Center | | | St. Albert, Council Bluffs | 303 | 1A | St. Albert, Council Bluffs | Missouri Valley
| | Sumner-Fredericksburg | 178 | 1A | Sumner-Fredericksburg | | | Test School 1 | 0 | | Test School 1 | | | Tri-Center, Neola | 156 | 1A | Tri-Center, Neola | | | Tripoli | 102 | 1A | Tripoli | | | Urbandale | 986 | 2A | Urbandale | | | Valley, West Des Moines | 2192 | 3A | Valley, West Des Moines | | | Vinton-Shellsburg | 352 | 1A | Vinton-Shellsburg | | | Wahlert, Dubuque | 426 | 1A | Wahlert, Dubuque | Marquette Catholic, Bellevue
| | Washington | 382 | 1A | Washington | | | Waterloo West | 1793 | 3A | Waterloo, West | Waterloo Christian School
Hudson
Union, La Porte City
| | Waterloo, East | 793 | 2A | Waterloo, East | | | Waukee | 3053 | 3A | Waukee | Perry
ADM, Adel
| | Waukon | 274 | 1A | Waukon | | | Waverly-Shell Rock | 691 | 2A | Waverly-Shell Rock | Nashua-Plainfield
| | West Burlington-Notre Dame | 226 | 1A | West Burlington | | | West Central Valley | 190 | 1A | West Central Valley, Stuart | | | West Delaware, Manchester | 375 | 1A | West Delaware, Manchester | | | West Sioux-MOC-FV-Akron-Westfield | 326 | 2A | West Sioux, Hawarden | Akron-Westfield
| | Xavier, Cedar Rapids | 454 | 2A | Xavier, Cedar Rapids | |
|