| Team Name | Enroll
-ment | Class | Head School | Co-oping Schools
(if Applicable) | | ADM, Adel | 566 | 2A | ADM, Adel | Van Meter
| | AHSTW, Avoca | 166 | 1A | AHSTW, Avoca | | | Albia | 285 | 1A | Albia | | | Ames | 1026 | 3A | Ames | | | Anamosa | 311 | 1A | Anamosa | | | Ankeny | 1321 | 3A | Ankeny | Ankeny Christian Academy
| | Ankeny Centennial | 1269 | 3A | Ankeny Centennial | | | Assumption, Davenport | 350 | 2A | Assumption, Davenport | | | Atlantic | 300 | 1A | Atlantic | | | Ballard | 403 | 2A | Ballard | | | Beckman Catholic, Dyersville | 203 | 1A | Beckman Catholic, Dyersville | | | Belmond-Klemme | 174 | 1A | Belmond-Klemme | | | Benton Community | 361 | 2A | Benton Community | | | Bettendorf | 1231 | 3A | Bettendorf | Morning Star Academy, Bettendorf
Rivermont Collegiate, Bettendorf
| | Bishop Heelan Catholic, Sioux City | 363 | 2A | Bishop Heelan Catholic, Sioux City | | | Bondurant-Farrar | 436 | 2A | Bondurant-Farrar | | | Boone | 628 | 2A | Boone | Ogden
| | Burlington | 800 | 3A | Burlington | | | Carlisle | 464 | 2A | Carlisle | | | Carroll | 493 | 2A | Carroll | Ar-We-Va, Westside
Glidden-Ralston
| | Cedar Falls | 1238 | 3A | Cedar Falls | Valley Lutheran, Cedar Falls
| | Cedar Rapids, Jefferson | 1191 | 3A | Cedar Rapids, Jefferson | | | Cedar Rapids, Kennedy | 1395 | 3A | Cedar Rapids, Kennedy | | | Cedar Rapids, Washington | 989 | 3A | Cedar Rapids, Washington | | | Cedar Valley Christian | 187 | 1A | Cedar Valley Christian | Alburnett
| | Center Point-Urbana | 369 | 2A | Center Point-Urbana | | | Centerville | 361 | 2A | Centerville | Moulton-Udell
| | Central DeWitt | 486 | 2A | Central DeWitt | Calamus-Wheatland
| | Central Iowa United | 502 | 2A | Collins-Maxwell | Baxter
Colo-Nesco
West Marshall, State Center
| | Central Lee, Donnellson | 232 | 1A | Central Lee, Donnellson | | | Chariton | 331 | 1A | Chariton | Mormon Trail, Garden Grove
| | Charles City | 394 | 2A | Charles City | | | Clarinda Academy | 111 | 1A | Clarinda Academy | | | Clarke, Osceola | 287 | 1A | Clarke, Osceola | | | Clear Creek Amana | 620 | 2A | Clear Creek-Amana | Belle Plaine
| | Clear Lake | 295 | 1A | Clear Lake | | | Clinton | 955 | 3A | Clinton | Camanche
| | Columbus Catholic, Waterloo | 294 | 1A | Columbus Catholic, Waterloo | Don Bosco, Gilbertville
| | Columbus/WMU | 269 | 1A | Columbus Community, Columbus Junction | Winfield-Mt. Union
| | Council Bluffs, Abraham Lincoln | 980 | 3A | Council Bluffs, Abraham Lincoln | | | Council Bluffs, Thomas Jefferson | 951 | 3A | Council Bluffs, Thomas Jefferson | | | Creston | 352 | 2A | Creston | | | Dallas Center-Grimes | 641 | 2A | Dallas Center-Grimes | | | Danville | 282 | 1A | Danville | New London
| | Davenport, Central | 1133 | 3A | Davenport, Central | | | Davenport, North | 913 | 3A | Davenport, North | | | Davenport, West | 1164 | 3A | Davenport, West | | | Decorah | 454 | 2A | Decorah | | | Denison-Schleswig | 614 | 2A | Denison-Schleswig | | | Denver | 274 | 1A | Denver | Tripoli
| | Des Moines Christian | 229 | 1A | Des Moines Christian | | | Des Moines East | 1951 | 3A | Des Moines, East | Saydel
| | Des Moines, Abraham Lincoln | 1793 | 3A | Des Moines, Abraham Lincoln | | | Des Moines, Hoover | 804 | 3A | Des Moines, Hoover | | | Des Moines, North | 1095 | 3A | Des Moines, North | | | Des Moines, Roosevelt | 1533 | 3A | Des Moines, Roosevelt | | | Dowling Catholic, West Des Moines | 1076 | 3A | Dowling Catholic, West Des Moines | | | Dubuque, Hempstead | 1293 | 3A | Dubuque, Hempstead | | | Dubuque, Senior | 1138 | 3A | Dubuque, Senior | | | East Sac County | 180 | 1A | East Sac County | | | Epworth, Western Dubuque | 842 | 3A | Epworth, Western Dubuque | Cascade, Western Dubuque
| | Fairfield High School | 419 | 2A | Fairfield | Maharishi School
| | Fort Dodge | 1238 | 3A | Fort Dodge | Southeast Valley, Gowrie
St. Edmond, Fort Dodge
| | Fort Madison | 476 | 2A | Fort Madison | Holy Trinity Catholic, Fort Madison
| | Garner-Hayfield-Ventura | 219 | 1A | Garner-Hayfield-Ventura | | | GCGR, Gladbrook-Reinbeck | 256 | 1A | Gladbrook-Reinbeck | Grundy Center
| | Gilbert | 366 | 2A | Gilbert | | | Glenwood | 663 | 2A | Glenwood | Fremont-Mills, Tabor
East Mills
| | Grand View Christian School | 84 | 1A | Grand View Christian School | | | Greene County | 341 | 1A | Greene County | Paton-Churdan
| | Grinnell | 493 | 2A | Grinnell | BGM, Brooklyn
| | Hampton-Dumont-CAL | 315 | 1A | Hampton-Dumont-CAL | | | Harlan | 395 | 2A | Harlan Community | | | Highland, Riverside | 139 | 1A | Highland, Riverside | | | Hillcrest Academy | 56 | 1A | Hillcrest Academy | | | Holy Trinity Catholic, Fort Madison | 76 | 1A | Holy Trinity Catholic, Fort Madison | | | Hudson | 454 | 2A | Hudson | Union, La Porte City
| | Humboldt | 362 | 2A | Humboldt | | | Independence | 706 | 3A | Independence | East Buchanan, Winthrop
Jesup
| | Indianola | 978 | 3A | Indianola | Martensdale-St. Marys
| | Iowa City, City High | 1277 | 3A | Iowa City, City High | Lone Tree
| | Iowa City, Liberty | 846 | 3A | Iowa City, Liberty | | | Iowa City, West | 1088 | 3A | Iowa City, West | | | Iowa Falls-Alden-AGWSR | 418 | 2A | Iowa Falls-Alden | AGWSR, Ackley
| | Johnston | 1858 | 3A | Johnston | Woodward-Granger
| | Keokuk | 384 | 2A | Keokuk | | | Knoxville | 560 | 2A | Knoxville | Pleasantville
| | Kuemper Catholic, Carroll | 228 | 1A | Kuemper Catholic, Carroll | | | Lewis Central | 697 | 2A | Lewis Central | | | Linn-Mar, Marion | 1667 | 3A | Linn-Mar, Marion | | | Logan-Magnolia | 148 | 1A | Logan-Magnolia | | | Maquoketa | 491 | 2A | Maquoketa | Easton Valley
| | Marion | 519 | 2A | Marion | | | Marquette Catholic, Bellevue | 235 | 1A | Marquette Catholic, Bellevue | Bellevue
| | Marshalltown | 1093 | 3A | Marshalltown | | | Mason City | 916 | 3A | Mason City | Newman Catholic, Mason City
| | Mediapolis | 179 | 1A | Mediapolis | | | Mid-Prairie, Wellman | 268 | 1A | Mid-Prairie, Wellman | | | Missouri Valley | 186 | 1A | Missouri Valley | | | MOC-Floyd Valley | 315 | 1A | MOC-Floyd Valley | | | Monticello | 275 | 1A | Monticello | | | Mount Pleasant | 427 | 2A | Mount Pleasant | | | Mount Vernon | 437 | 2A | Mount Vernon | Lisbon
| | Muscatine | 1679 | 3A | Muscatine | Durant
Wilton
Louisa-Muscatine
| | Nevada | 330 | 1A | Nevada | | | Newton | 651 | 2A | Newton | | | North Fayette Valley | 230 | 1A | North Fayette Valley | | | North Polk, Alleman | 357 | 2A | North Polk, Alleman | | | North Scott, Eldridge | 705 | 3A | North Scott, Eldridge | | | Norwalk | 641 | 2A | Norwalk | | | Notre Dame, Burlington | 320 | 1A | Notre Dame, Burlington | West Burlington
| | Oskaloosa | 476 | 2A | Oskaloosa | | | Ottumwa | 1171 | 3A | Ottumwa | Eddyville-Blakesburg-Fremont
| | Panorama | 394 | 2A | Panorama, Panora | ACGC
| | PCM, Monroe | 239 | 1A | PCM, Monroe | | | Pella | 520 | 2A | Pella | | | Pella Christian | 196 | 1A | Pella Christian | | | Perry | 576 | 2A | Perry | Madrid
| | Pleasant Valley | 1073 | 3A | Pleasant Valley | | | Postville | 172 | 1A | Postville | | | Prairie, Cedar Rapids | 1283 | 3A | Prairie, Cedar Rapids | | | Prince of Peace Catholic | 247 | 1A | Prince of Peace Catholic, Clinton | Northeast, Goose Lake
| | Red Oak | 229 | 1A | Red Oak | | | Regina, Iowa City | 163 | 1A | Regina, Iowa City | | | Riverside, Oakland | 118 | 1A | Riverside, Oakland | | | Scattergood Friends | 23 | 1A | Scattergood Friends | | | Sergeant Bluff-Luton | 377 | 2A | Sergeant Bluff-Luton | | | Sheldon Sibley-Ocheyedan | 424 | 2A | Sheldon | Sibley-Ocheyedan
| | Sioux Center | 273 | 1A | Sioux Center | | | Sioux City, East | 1092 | 3A | Sioux City, East | | | Sioux City, North | 1159 | 3A | Sioux City, North | | | Sioux City, West | 936 | 3A | Sioux City, West | | | Solon | 350 | 2A | Solon | | | South Tama County, Tama | 532 | 2A | South Tama County, Tama | East Marshall, LeGrand
| | Southeast Polk | 1541 | 3A | Southeast Polk | | | Spencer | 683 | 2A | Spencer | Emmetsburg
| | Spirit Lake | 556 | 2A | Spirit Lake | Harris-Lake Park
Okoboji, Milford
| | St. Albert, Council Bluffs | 125 | 1A | St. Albert, Council Bluffs | | | Storm Lake | 601 | 2A | Storm Lake | St. Mary's, Storm Lake
| | Tipton | 249 | 1A | Tipton | | | Treynor | 296 | 1A | Treynor | Fremont-Mills, Tabor
| | Tri-Center, Neola | 163 | 1A | Tri-Center, Neola | | | Underwood | 194 | 1A | Underwood | | | Unity Christian, Orange City | 213 | 1A | Unity Christian, Orange City | | | Urbandale | 983 | 3A | Urbandale | | | Valley, West Des Moines | 2201 | 3A | Valley, West Des Moines | | | Vinton-Shellsburg | 336 | 1A | Vinton-Shellsburg | | | Wahlert, Dubuque | 368 | 2A | Wahlert, Dubuque | | | Wapello | 157 | 1A | Wapello | | | Washington | 661 | 2A | Washington | Pekin
WACO, Wayland
| | Waterloo, East | 899 | 3A | Waterloo, East | Dunkerton
| | Waterloo, West | 1514 | 3A | Waterloo, West | Waterloo Christian School
Wapsie Valley, Fairbank
| | Waukee | 2147 | 3A | Waukee | | | Waverly-Shell Rock | 607 | 2A | Waverly-Shell Rock | Janesville
| | Webster City | 414 | 2A | Webster City |
| | West Branch | 183 | 1A | West Branch | | | West Central Valley, Stuart | 183 | 1A | West Central Valley, Stuart |
| | West Delaware, Manchester | 518 | 2A | West Delaware, Manchester | Maquoketa Valley, Delhi
| | West Liberty | 282 | 1A | West Liberty | | | West Sioux, Hawarden | 182 | 1A | West Sioux, Hawarden | | | Western Christian, Hull | 197 | 1A | Western Christian, Hull | | | Williamsburg | 259 | 1A | Williamsburg | | | Winterset | 550 | 2A | Winterset | Earlham
| | Xavier, Cedar Rapids | 499 | 2A | Xavier, Cedar Rapids | |
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